Phenotypes of the Regan isoenzyme and identity between the placental D-variant and the Nagao isoenzyme.
نویسندگان
چکیده
We observed an unexpectedly high incidence of the D-variant placenta! phenotype in Regan isoenzyme-positive sera of cancer patients-. Such D-variant-positive sera exhibit a high degree of inhibition by L-leucine, a phenomenon earlier associated with the Nagao isoenzyme. Unlike normal pla centae, a diffuse phenotype of heat-stable alkaline phosphatase is usually noted in cancer sera containing Nagao isoenzyme.
منابع مشابه
Studies of the Regan alkaline phosphatase isoenzyme in plasma from a lung-carcinoma patient.
We compared an alkaline phosphatase (EC 3.1.3.1) isoenzyme from a plasma sample of a patient with carcinoma of the lung, who was suspected of having the Regan-type isoenzyme, with placental serum alkaline phosphatase and with tissue preparations from placenta, liver, bone, and intestine. The placental and Regan phosphatases responded similarly to heat treatment and modification by various inhib...
متن کامل[Alkaline phosphatase as a tumor marker].
Applications and limitations of alkaline phosphatase as a tumor marker are discussed. This review focuses on three characteristic isoenzymes that have been found in the serum of cancer patients: (1) the Regan isoenzyme in bronchogenic carcinoma; (2) the Nagao isoenzyme in pleuritis carcinomatosa; and (3) the hepatoma alkaline phosphatase. Tumor variants of alkaline phosphatase are classified ba...
متن کاملCharacterization of Two Hela Sublines: Tcrc-1 Produces Regan Isoenzyme and Tcrc-2, Non-regan Isoenzyme
HeLa Cells have been found to produce a placental form of alkaline phosphatase (1, 8, 17) which is very similar to the Regan isoenzyme, a carcinoplacental antigen found in cancer patients (7-9) . In order to investigate the nature of gene expression of carcinoplacental proteins, HeLa cells were cloned, and a subline (HeLa TCRC-1), monophenotypic for the Regan isoenzyme was selected . The proper...
متن کاملComparison of Human Alkaline Phosphatase Isoenzymes
The structural relationships among human alkaline phosphatase isoenzymes from placenta, bone, kidney, liver and intestine were investigated by using three criteria. 1. Immunochemical characterization by using monospecific antisera prepared against either the placental isoenzyme or the liver isoenzyme distinguishes two antigenic groups: bone, kidney and liver isoenzymes cross-react with anti-(li...
متن کاملPURIFICATION AND SOME PARTIAL CHARACTERIZATION OF PEROXIDASE ISOENZYME FROM BRASSICA OLERACEA CAPITATA L.
Acetone fractionated peroxidase from crude extract of Brassica oleracea leaves (Cabbage) was purified in three steps on chromatographic columns, using Sp-Sepharose, DEAE-Sepharose and Con A-Sepharose. The specific activity of purified main isoenzyme (BOC-POD) is 1887 u/mg protein with RZ: 3.1, which is 172 times more than the RZ of crude extract with 4.3% recovery. The molecular weight of BOC-P...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Cancer research
دوره 33 7 شماره
صفحات -
تاریخ انتشار 1973